Journal: BMC Complementary and Alternative Medicine

Article Title: Spermicidal and anti- Trichomonas vaginalis activity of Brazilian Sapindus saponaria

doi: 10.1186/1472-6882-13-196

Figure Lengend Snippet: Susceptibility of Trichomonas vaginalis to water-ethanol (WE) and butanolic (BE) extracts, and saponins (SP) of Sapindus saponaria

Article Snippet: The compounds were effective against Trichomonas vaginalis (Minimum Inhibitory Concentration = 0.156 mg/mL for WE and BE, and 0.078 mg/mL for SP against a clinical strain (CS); and 0.312, 0.156 and 0.078 mg/mL for WE, BE and SP, respectively, against an ATCC strain).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Thanatin: A Promising Antimicrobial Peptide Targeting the Achilles’ Heel of Multidrug-Resistant Bacteria

doi: 10.3390/ijms25179496

Figure Lengend Snippet: Antimicrobial activity of thanatin, S-thanatin, and A-thanatin against Gram-positive bacteria.

Article Snippet: Particularly noteworthy is its strong antimicrobial effect against Enterococcus faecalis ATCC 29212 and Streptococcus pyogenes ATCC 19615, with MIC values of 0.5 μM [ ].

Techniques: Activity Assay, Bacteria

Journal: Pharmaceutics

Article Title: In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics

doi: 10.3390/pharmaceutics12121217

Figure Lengend Snippet: Antimicrobial activity of assembled (PNPs) and unassembled soluble proteins (UPs) in vitro. ( A ) Effect on cell viability of increasing amounts of GWH1-GFP and GWH1-IFN-γ against E. coli and ( B ) S. aureus ( C ). Dynamic light scattering (DLS) analysis showing the nanoparticle disassembling of GWH1-GFP in presence of 0.2% N-lauroylsarcosine. ( D ) Comparative effect on cell viability of disassembled (promoted in presence of 0.2% N-lauroylsarcosine) and assembled GWH1-GFP against E. coli in vitro. IFN-γ and GFP were used as controls. Significant results are shown as * p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001, multiple t test. All experiments were performed in duplicates. Bars represent the the mean ± standard deviation.

Article Snippet: The effect of the different antimicrobial candidates was evaluated against E. coli ATCC 25922 and Staphylococcus aureus ATCC 29737.

Techniques: Activity Assay, In Vitro, Standard Deviation

Journal: Pharmaceutics

Article Title: In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics

doi: 10.3390/pharmaceutics12121217

Figure Lengend Snippet: Growth kinetics of E. coli in the mouse mammary gland at 6, 12 and 24 h after the administration of 100 CFU through the teat channel. The bacterial loads for each time point were evaluated in 8 individual mammary glands (4 mice).Bars represent the mean ± standard deviation.

Article Snippet: The effect of the different antimicrobial candidates was evaluated against E. coli ATCC 25922 and Staphylococcus aureus ATCC 29737.

Techniques: Standard Deviation

Journal: Pharmaceutics

Article Title: In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics

doi: 10.3390/pharmaceutics12121217

Figure Lengend Snippet: Bacterial loads ( E. coli CFU/g of gland log10) measured after the administration of therapeutic and non-therapeutic protein formats into infected mammary glands. ( A ) Effect of unassembled protein forms (UPs) and assembled protein-only nanoparticles (PNPs) 8 h after the challenge with 100 CFU of E. coli . ( B ) Effect of the inclusion body (IB) format 8 h after the challenge with 100 CFU of E. coli . In all cases, 60 µM of the different protein formats were administered just after the infection with E. coli . Sodium bicarbonate buffer (166 mmol/L NaHCO 3 , 333 mmol/L NaCl) and GFP were used as controls. The middle bars indicate median values for each group of glands (at least 6 glands) whereas the boxes specify quartiles Q1-Q3. Experiments were performed with at least three independent replicates. Significant results are shown as ** p ≤ 0.01 and *** p ≤ 0.001, one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test.

Article Snippet: The effect of the different antimicrobial candidates was evaluated against E. coli ATCC 25922 and Staphylococcus aureus ATCC 29737.

Techniques: Infection